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Hichrom Limited nonpolar ec-5 capillary column
Nonpolar Ec 5 Capillary Column, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capillary+column+ec-5/ec+1+capillary+column/pmc06091922-86-14-24
Average 90 stars, based on 1 article reviews
nonpolar ec-5 capillary column - by Bioz Stars, 2026-09
90/100 stars

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Article Title: An Alternative Pathway of Oleate β-Oxidation in Escherichia coli Involving the Hydrolysis of a Dead End Intermediate by a Thioesterase
Article Snippet: Aliquots of 1 l of the fatty acid methyl esters were injected at 250 °C into a GC/MS instrument (Shimadzu Scientific Instruments) consisting of a gas chromatograph (model GC-17A) interphased with a mass spectrometer (QP5000) and equipped with a capillary column (30 m; inner diameter, 0.25 mm; film thickness, 0.25 m; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Direct electrochemical reduction of a bromo-propargyloxy ester at vitreous carbon cathodes in dimethylformamide
Article Snippet: Cyclic voltammograms for the reduction of ethyl 2-bromo-3-(30; 40-dimethoxyphenyl)-3-(propargyloxy)propanoate (1) at glassy carbon electrodes in dimethylformamide containing tetraalkylammonium salts exhibit three prominent waves corresponding to cleavage of the carbon–bromine bond and to subsequent reduction of ethyl trans-3-(30; 40-dimethoxyphenyl)-prop-2-enoate (4).. Controlled-potential electrolyses of 1 at potentials corresponding to reduction of the carbon–bromine bond afford 4 as the major product with an average yield of 56%.. In the presence of a proton donor (1,1,1,3,3,3-hexafluoro-2-propanol), the quantity of 4 decreases slightly, and 2-(30; 40-dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methyl-2,5-dihydrofuran (3) is obtained in moderate amount ( 26%).

Article Title: Electroreductive Radical Cyclization of Ethyl 2‐Bromo‐3‐allyloxy‐ and‐3‐(propargyloxy)propanoates Catalyzed by (Tetramethylcyclam)nickel(<scp>I</scp>) Electrogenerated at Carbon Cathodes in Dimethylformamide
Article Snippet: Reductive intramolecular cyclization of ethyl 2-bromo-3(3 ,4 -methylenedioxyphenyl)-3-(propargyloxy)propanoate (1) and ethyl 3-allyloxy-2-bromo-3-(3 ,4 -dimethoxyphenyl)propanoate (2) promoted by (1,4,8,11-tetramethyl-1,4,8,11tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy-carbon cathodes in dimethylformamide containing tetraalkylammonium salts, has been investigated.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 and 2 reveal that [Ni(tmc)]+ catalytically reduces these two compounds at potentials significantly more positive than those required for direct reduction of the bromo

Article Title: Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation.
Article Snippet: When Escherichia coli is grown on oleic acid as the sole carbon source, most of this fatty acid is completely degraded by -oxidation.. However, approximately 10% of the oleic acid is only partially degraded to 3,5-cis-tetradecadienoyl-CoA, which is hydrolyzed to 3,5-cis-tetradecadienoic acid and released into the growth medium.. An investigation of thioesterases involved in this novel pathway of -oxidation led to the identification of a new thioesterase (thioesterase III) that is induced by growth of E. coli on oleic acid.

Article Title: Oleate β-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase
Article Snippet: Aliquots of 1 μl of the fatty acid methyl esters were injected at 250°C into a gas chromatograph (Shimadzu Scientific Instrument, model GC-17A) interphased with a mass spectrometer (QP-5000) and equipped with a capillary column (30m; inner diameter 0.25 mm; film thickness, 0.25 μm; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Electroreductive intramolecular cyclization of a bromo propargyloxy ester catalyzed by nickel(i) tetramethylcyclam electrogenerated at carbon cathodes in dimethylformamide.
Article Snippet: Cyclic voltammetry and controlled-potential electrolysis have been employed to investigate and characterize the reductive intramolecular cyclization of ethyl 2-bromo-3-(3′,4′-dimethoxyphenyl)3-(propargyloxy)propanoate (1) promoted by (1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy carbon cathodes in dimethylformamide containing tetraalkylammonium salts.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 reveal that [Ni(tmc)]+ catalytically reduces 1 at potentials more positive than those required for direct reduction of 1.. During controlled-potential electrolyses of solutions containing [Ni(tmc)]2+ and 1, catalytic reduction of the latter proceeds via one-electron cleavage of the carbonbromine bond to form a radical intermediate that undergoes cyclization to afford 2-(3′,4′dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methylenetetrahydrofuran (2).

Mass Spectrometry:

Article Title: An Alternative Pathway of Oleate β-Oxidation in Escherichia coli Involving the Hydrolysis of a Dead End Intermediate by a Thioesterase
Article Snippet: Aliquots of 1 l of the fatty acid methyl esters were injected at 250 °C into a GC/MS instrument (Shimadzu Scientific Instruments) consisting of a gas chromatograph (model GC-17A) interphased with a mass spectrometer (QP5000) and equipped with a capillary column (30 m; inner diameter, 0.25 mm; film thickness, 0.25 m; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Direct electrochemical reduction of a bromo-propargyloxy ester at vitreous carbon cathodes in dimethylformamide
Article Snippet: Cyclic voltammograms for the reduction of ethyl 2-bromo-3-(30; 40-dimethoxyphenyl)-3-(propargyloxy)propanoate (1) at glassy carbon electrodes in dimethylformamide containing tetraalkylammonium salts exhibit three prominent waves corresponding to cleavage of the carbon–bromine bond and to subsequent reduction of ethyl trans-3-(30; 40-dimethoxyphenyl)-prop-2-enoate (4).. Controlled-potential electrolyses of 1 at potentials corresponding to reduction of the carbon–bromine bond afford 4 as the major product with an average yield of 56%.. In the presence of a proton donor (1,1,1,3,3,3-hexafluoro-2-propanol), the quantity of 4 decreases slightly, and 2-(30; 40-dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methyl-2,5-dihydrofuran (3) is obtained in moderate amount ( 26%).

Article Title: Electroreductive Radical Cyclization of Ethyl 2‐Bromo‐3‐allyloxy‐ and‐3‐(propargyloxy)propanoates Catalyzed by (Tetramethylcyclam)nickel(<scp>I</scp>) Electrogenerated at Carbon Cathodes in Dimethylformamide
Article Snippet: Reductive intramolecular cyclization of ethyl 2-bromo-3(3 ,4 -methylenedioxyphenyl)-3-(propargyloxy)propanoate (1) and ethyl 3-allyloxy-2-bromo-3-(3 ,4 -dimethoxyphenyl)propanoate (2) promoted by (1,4,8,11-tetramethyl-1,4,8,11tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy-carbon cathodes in dimethylformamide containing tetraalkylammonium salts, has been investigated.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 and 2 reveal that [Ni(tmc)]+ catalytically reduces these two compounds at potentials significantly more positive than those required for direct reduction of the bromo

Article Title: Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation.
Article Snippet: When Escherichia coli is grown on oleic acid as the sole carbon source, most of this fatty acid is completely degraded by -oxidation.. However, approximately 10% of the oleic acid is only partially degraded to 3,5-cis-tetradecadienoyl-CoA, which is hydrolyzed to 3,5-cis-tetradecadienoic acid and released into the growth medium.. An investigation of thioesterases involved in this novel pathway of -oxidation led to the identification of a new thioesterase (thioesterase III) that is induced by growth of E. coli on oleic acid.

Article Title: Oleate β-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase
Article Snippet: Aliquots of 1 μl of the fatty acid methyl esters were injected at 250°C into a gas chromatograph (Shimadzu Scientific Instrument, model GC-17A) interphased with a mass spectrometer (QP-5000) and equipped with a capillary column (30m; inner diameter 0.25 mm; film thickness, 0.25 μm; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Electroreductive intramolecular cyclization of a bromo propargyloxy ester catalyzed by nickel(i) tetramethylcyclam electrogenerated at carbon cathodes in dimethylformamide.
Article Snippet: Cyclic voltammetry and controlled-potential electrolysis have been employed to investigate and characterize the reductive intramolecular cyclization of ethyl 2-bromo-3-(3′,4′-dimethoxyphenyl)3-(propargyloxy)propanoate (1) promoted by (1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy carbon cathodes in dimethylformamide containing tetraalkylammonium salts.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 reveal that [Ni(tmc)]+ catalytically reduces 1 at potentials more positive than those required for direct reduction of 1.. During controlled-potential electrolyses of solutions containing [Ni(tmc)]2+ and 1, catalytic reduction of the latter proceeds via one-electron cleavage of the carbonbromine bond to form a radical intermediate that undergoes cyclization to afford 2-(3′,4′dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methylenetetrahydrofuran (2).

Gas Chromatography-Mass Spectrometry:

Article Title: An Alternative Pathway of Oleate β-Oxidation in Escherichia coli Involving the Hydrolysis of a Dead End Intermediate by a Thioesterase
Article Snippet: Aliquots of 1 l of the fatty acid methyl esters were injected at 250 °C into a GC/MS instrument (Shimadzu Scientific Instruments) consisting of a gas chromatograph (model GC-17A) interphased with a mass spectrometer (QP5000) and equipped with a capillary column (30 m; inner diameter, 0.25 mm; film thickness, 0.25 m; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Direct electrochemical reduction of a bromo-propargyloxy ester at vitreous carbon cathodes in dimethylformamide
Article Snippet: Cyclic voltammograms for the reduction of ethyl 2-bromo-3-(30; 40-dimethoxyphenyl)-3-(propargyloxy)propanoate (1) at glassy carbon electrodes in dimethylformamide containing tetraalkylammonium salts exhibit three prominent waves corresponding to cleavage of the carbon–bromine bond and to subsequent reduction of ethyl trans-3-(30; 40-dimethoxyphenyl)-prop-2-enoate (4).. Controlled-potential electrolyses of 1 at potentials corresponding to reduction of the carbon–bromine bond afford 4 as the major product with an average yield of 56%.. In the presence of a proton donor (1,1,1,3,3,3-hexafluoro-2-propanol), the quantity of 4 decreases slightly, and 2-(30; 40-dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methyl-2,5-dihydrofuran (3) is obtained in moderate amount ( 26%).

Article Title: Electroreductive Radical Cyclization of Ethyl 2‐Bromo‐3‐allyloxy‐ and‐3‐(propargyloxy)propanoates Catalyzed by (Tetramethylcyclam)nickel(<scp>I</scp>) Electrogenerated at Carbon Cathodes in Dimethylformamide
Article Snippet: Reductive intramolecular cyclization of ethyl 2-bromo-3(3 ,4 -methylenedioxyphenyl)-3-(propargyloxy)propanoate (1) and ethyl 3-allyloxy-2-bromo-3-(3 ,4 -dimethoxyphenyl)propanoate (2) promoted by (1,4,8,11-tetramethyl-1,4,8,11tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy-carbon cathodes in dimethylformamide containing tetraalkylammonium salts, has been investigated.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 and 2 reveal that [Ni(tmc)]+ catalytically reduces these two compounds at potentials significantly more positive than those required for direct reduction of the bromo

Article Title: Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation.
Article Snippet: When Escherichia coli is grown on oleic acid as the sole carbon source, most of this fatty acid is completely degraded by -oxidation.. However, approximately 10% of the oleic acid is only partially degraded to 3,5-cis-tetradecadienoyl-CoA, which is hydrolyzed to 3,5-cis-tetradecadienoic acid and released into the growth medium.. An investigation of thioesterases involved in this novel pathway of -oxidation led to the identification of a new thioesterase (thioesterase III) that is induced by growth of E. coli on oleic acid.

Article Title: Oleate β-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase
Article Snippet: Aliquots of 1 μl of the fatty acid methyl esters were injected at 250°C into a gas chromatograph (Shimadzu Scientific Instrument, model GC-17A) interphased with a mass spectrometer (QP-5000) and equipped with a capillary column (30m; inner diameter 0.25 mm; film thickness, 0.25 μm; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Electroreductive intramolecular cyclization of a bromo propargyloxy ester catalyzed by nickel(i) tetramethylcyclam electrogenerated at carbon cathodes in dimethylformamide.
Article Snippet: Cyclic voltammetry and controlled-potential electrolysis have been employed to investigate and characterize the reductive intramolecular cyclization of ethyl 2-bromo-3-(3′,4′-dimethoxyphenyl)3-(propargyloxy)propanoate (1) promoted by (1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy carbon cathodes in dimethylformamide containing tetraalkylammonium salts.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 reveal that [Ni(tmc)]+ catalytically reduces 1 at potentials more positive than those required for direct reduction of 1.. During controlled-potential electrolyses of solutions containing [Ni(tmc)]2+ and 1, catalytic reduction of the latter proceeds via one-electron cleavage of the carbonbromine bond to form a radical intermediate that undergoes cyclization to afford 2-(3′,4′dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methylenetetrahydrofuran (2).

Pyrolysis Gas Chromatography:

Article Title: An Alternative Pathway of Oleate β-Oxidation in Escherichia coli Involving the Hydrolysis of a Dead End Intermediate by a Thioesterase
Article Snippet: Aliquots of 1 l of the fatty acid methyl esters were injected at 250 °C into a GC/MS instrument (Shimadzu Scientific Instruments) consisting of a gas chromatograph (model GC-17A) interphased with a mass spectrometer (QP5000) and equipped with a capillary column (30 m; inner diameter, 0.25 mm; film thickness, 0.25 m; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Direct electrochemical reduction of a bromo-propargyloxy ester at vitreous carbon cathodes in dimethylformamide
Article Snippet: Cyclic voltammograms for the reduction of ethyl 2-bromo-3-(30; 40-dimethoxyphenyl)-3-(propargyloxy)propanoate (1) at glassy carbon electrodes in dimethylformamide containing tetraalkylammonium salts exhibit three prominent waves corresponding to cleavage of the carbon–bromine bond and to subsequent reduction of ethyl trans-3-(30; 40-dimethoxyphenyl)-prop-2-enoate (4).. Controlled-potential electrolyses of 1 at potentials corresponding to reduction of the carbon–bromine bond afford 4 as the major product with an average yield of 56%.. In the presence of a proton donor (1,1,1,3,3,3-hexafluoro-2-propanol), the quantity of 4 decreases slightly, and 2-(30; 40-dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methyl-2,5-dihydrofuran (3) is obtained in moderate amount ( 26%).

Article Title: Electroreductive Radical Cyclization of Ethyl 2‐Bromo‐3‐allyloxy‐ and‐3‐(propargyloxy)propanoates Catalyzed by (Tetramethylcyclam)nickel(<scp>I</scp>) Electrogenerated at Carbon Cathodes in Dimethylformamide
Article Snippet: Reductive intramolecular cyclization of ethyl 2-bromo-3(3 ,4 -methylenedioxyphenyl)-3-(propargyloxy)propanoate (1) and ethyl 3-allyloxy-2-bromo-3-(3 ,4 -dimethoxyphenyl)propanoate (2) promoted by (1,4,8,11-tetramethyl-1,4,8,11tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy-carbon cathodes in dimethylformamide containing tetraalkylammonium salts, has been investigated.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 and 2 reveal that [Ni(tmc)]+ catalytically reduces these two compounds at potentials significantly more positive than those required for direct reduction of the bromo

Article Title: Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation.
Article Snippet: When Escherichia coli is grown on oleic acid as the sole carbon source, most of this fatty acid is completely degraded by -oxidation.. However, approximately 10% of the oleic acid is only partially degraded to 3,5-cis-tetradecadienoyl-CoA, which is hydrolyzed to 3,5-cis-tetradecadienoic acid and released into the growth medium.. An investigation of thioesterases involved in this novel pathway of -oxidation led to the identification of a new thioesterase (thioesterase III) that is induced by growth of E. coli on oleic acid.

Article Title: Oleate β-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase
Article Snippet: Aliquots of 1 μl of the fatty acid methyl esters were injected at 250°C into a gas chromatograph (Shimadzu Scientific Instrument, model GC-17A) interphased with a mass spectrometer (QP-5000) and equipped with a capillary column (30m; inner diameter 0.25 mm; film thickness, 0.25 μm; EC-5; Alltech Associates Inc., Deerfield, IL).

Article Title: Electroreductive intramolecular cyclization of a bromo propargyloxy ester catalyzed by nickel(i) tetramethylcyclam electrogenerated at carbon cathodes in dimethylformamide.
Article Snippet: Cyclic voltammetry and controlled-potential electrolysis have been employed to investigate and characterize the reductive intramolecular cyclization of ethyl 2-bromo-3-(3′,4′-dimethoxyphenyl)3-(propargyloxy)propanoate (1) promoted by (1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane)nickel(I), [Ni(tmc)]+, electrogenerated at glassy carbon cathodes in dimethylformamide containing tetraalkylammonium salts.. Cyclic voltammograms for reduction of [Ni(tmc)]2+ in the presence of 1 reveal that [Ni(tmc)]+ catalytically reduces 1 at potentials more positive than those required for direct reduction of 1.. During controlled-potential electrolyses of solutions containing [Ni(tmc)]2+ and 1, catalytic reduction of the latter proceeds via one-electron cleavage of the carbonbromine bond to form a radical intermediate that undergoes cyclization to afford 2-(3′,4′dimethoxyphenyl)-3-(ethoxycarbonyl)-4-methylenetetrahydrofuran (2).



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